Stability of southern bean mosaic and carnation mottle viruses during the preservation by freeze-drying.

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Stability of Southern Bean Mosaic and Carnation Mottle Viruses during the Preservation by Freeze-drying

The infectivity of purified southern bean mosaic virus (SBMV) in 10mM sodium phosphate buffer, pH 7.0, decreased to 11% after freeze-drying. Such partially swollen virions were found to be markedly susceptible to RNase and sodium dodecyl sulfate (SDS). However, in the preparations supplemented with 0.5% lysine prior to freeze-drying the conformational changes of the virions were suppressed and ...

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Preservation of Lactic Acid Bacteria by Freeze-Drying

Lactic acid bacteria are widely used in the manufacture of fermented milk products, such as cheeses, sour cream butter, yoghurt and fermented milk beverages. These microorganisms may also be applied to the production of fermented meat products and silage. We may easily cultivate lactic acid bacteria in milk. However, it is a laborious work to preserve the organisms in liquid state because viabi...

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BACKGROUND Freeze-drying sperm has been developed as a new preservation method where liquid nitrogen is no longer necessary. An advantage of freeze-drying sperm is that it can be stored at 4 °C and transported at room temperature. Although the successful freeze-drying of sperm has been reported in a number of animals, the possibility of long-term preservation using this method has not yet been ...

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ژورنال

عنوان ژورنال: Japanese Journal of Phytopathology

سال: 1990

ISSN: 1882-0484,0031-9473

DOI: 10.3186/jjphytopath.56.47